Buffers

Buffers

Buffers maintain their pH when small amounts of strong acids or bases are added. In biology, buffer solutions are used to provide a constant pH environment for biochemical processes. The pH often changes the environments of tissues and cells and can alter the activity of biomolecules. Buffers maintain the correct pH for proteins and oligonucleotides, and are especially important for techniques like electrophoresis.

Commonly used buffers in life science research share some of these characteristics:

  • A pKa value between 6.0 and 8.0
  • High water solubility
  • Minimal changes due to temperature and concentration
  • Limited effects due to ionic or salt composition of the solution

Browse our comprehensive selection of buffers including common buffers tris(hydroxymethyl)aminomethane (tris) and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES).

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Sodium phosphate, dibasic anhydrous, USP

Sec-Sodium phosphate; Disodium hydrogen phosphate; Disodium phosphate; Sodium hydrogenphosphate
7558-79-4
Na2HPO4
141.98

Sodium phosphate dibasic anhydrous is a sodium salt of phosphoric acid. It is a white powder that is highly hygroscopic and water soluble.

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Tris, ultra pure, ≥99.9%

77-86-1
NH2C(CH2OH)3

Bis-Tris propane, ultrapure

Bis-Tris propane, ultrapure

Bis-Tris Propane, UltraPure

Bis-Tris propane, molecular biology reagent, ≥99%
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Tris, ultra pure, ≥99.9%

Tris-[hydroxymethyl]aminomethane; THAM; 2-Amino-2-(hydroxymethyl)- 1,3-propandiol; Tromethamine; Trometamol
77-86-1
NH2C(CH2OH)3
121.136 g/mol

Tris

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Tween® 20, molecular biology reagent

TWEEN 20 is a polyoxyethylene sorbitol ester.

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Tris base, molecular biology grade, >99.8%

C4H11NO3
121,14

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.

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TBE Buffer, 10x

TBE buffer maintains the structural integrity of nucleic acids and more suitable for their size analysis. TBE has a greater buffering capacity and will give sharper resolution. TBE is better suited for high-voltage (>150 V) electrophoresis because of its higher buffering capacity.

PBS 10x
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Water, DNase, RNase-free

Distilled water; hydrogen oxide; Deionized water; Purified water; Dihydrogen Monoxide; DHMO
7732-18-5
H2O
18.015 g/mol

Water

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Urea, ultra pure, ≥99%

Carbamide; Isourea; Carbonyldiamide; Carbonyldiamine; Ureophil; Carbamimidic acid; Pseudourea; Ureaphil; Urevert
57-13-6
CH4N2O
60.06

Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.

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TBE buffer

TBE buffer
Not applicable

Tris-Borate-Edta Buffer

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Tris hydrochloride, ultra pure, ≥99%

Tris(hydroxymethyl)aminomethane hydrochloride; TRIS HCl
1185-53-1
C4H11NO3 • HCl
157.594 g/mol

Tris Hydrochloride

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Glycine, electrophoresis grade

Aminoacetic acid; Gly; Aminoethanoic acid; Glycocoll; G
56-40-6
C2H5NO2
75.067 g/mol

Glycine

Ammonium sulfate

Ammonium sulfate

Sulfuric acid diammonium salt; Mascagnite
7783-20-2
(NH4)2SO4
132.134 g/mol

Ammonium Sulfate

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Tricine, electrophoresis grade, ≥99%

N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine
5704-04-1
C6H13NO5

Tricine

Sodium phosphate, monobasic, monohydrate, USP

Sodium phosphate, monobasic, monohydrate, USP

Monosodium phosphate; Sodium dihydrogen phosphate monohydrate
10049-21-5
NaH2PO4·H2O
137.991 g/mol

Sodium Phosphate Monobasic, Monohydrate

Ammonium acetate, 7.5 M solution

Ammonium acetate, 7.5 M solution

Acetic acid ammonium salt; Ammonium acetate solution
631-61-8
NH4C2H3O2 in water
77.083 g/mol

Ammonium Acetate, 7.5 M Solution

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Tris, USP grade

Trometamol; Tromethamine; Tris-[hydroxymethyl]aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol;THAM; Tris base
77-86-1
C4H11NO3
121.136 g/mol

Tris

Sodium phosphate, dibasic, anhydrous, 98.0 - 102.0%

Sodium phosphate, dibasic, anhydrous, 98.0 - 102.0%

Disodium hydrogen phosphate; Dibasic sodium phosphate; Disodium orthophosphate; Disodium phosphate; DSP; Phosphate of soda; Secondary sodium phosphate; Sodium hydrogenphosphate; sec-Sodium phosphate
7558-79-4
HNa2O4P
141.957 g/mol

Sodium Phosphate Dibasic

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